Open AccessQuantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA
Author(s) -
Alejandro Monserrat García-Alegría,
Iván Anduro-Corona,
Cinthia J. Pérez-Martínez,
María Alba Guadalupe Corella-Madueño,
María Lucila Rascón-Durán,
Humberto Astiazarán-García
Publication year - 2020
Publication title -
international journal of analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.352
H-Index - 16
eISSN - 1687-8779
pISSN - 1687-8760
DOI - 10.1155/2020/8896738
Subject(s) - dna , computational biology , dna profiling , microbiology and biotechnology , chromatography , biology , computer science , bioinformatics , chemistry , genetics
This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z -test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C).