Open AccessAnnexin V+ Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study
Author(s) -
Vibeke Bratseth,
Hanna Dis Margeirsdóttir,
Gemma ChivaBlanch,
Martin Heier,
Svein Solheim,
Harald Arnesen,
Knut DahlJørgensen,
Ingebjørg Seljeflot
Publication year - 2020
Publication title -
journal of diabetes research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.034
H-Index - 50
eISSN - 2314-6753
pISSN - 2314-6745
DOI - 10.1155/2020/7216863
Subject(s) - type 1 diabetes , medicine , type 2 diabetes , diabetes mellitus , glycemic , cohort , prospective cohort study , gastroenterology , endocrinology
Background Type 1 diabetes is a chronic disease including hyperglycemia and accelerated atherosclerosis, with high risk of micro- and macrovascular complications. Circulating microvesicles (cMVs) are procoagulant cell fragments shed during activation/apoptosis and discussed to be markers of vascular dysfunction and hypercoagulability. Limited knowledge exists on hypercoagulability in young diabetics. We aimed to investigate cMVs over a five-year period in children/adolescents with type 1 diabetes compared with controls and any associations with glycemic control and cardiovascular risk factors. We hypothesized increased shedding of cMVs in type 1 diabetes in response to vascular activation.Methods The cohort included type 1 diabetics ( n = 40) and healthy controls ( n = 40), mean age 14 years (range 11) at inclusion, randomly selected from the Norwegian Atherosclerosis and Childhood Diabetes (ACD) study. Citrated plasma was prepared and stored at -80°C until cMV analysis by flow cytometry.Results Comparable levels of Annexin V (AV + ) cMVs were observed at inclusion. At five-year follow-up, total AV + cMVs were significantly lower in subjects with type 1 diabetes compared with controls; however, no significant differences were observed after adjusting for covariates. In the type 1 diabetes group, the total AV + , tissue factor-expressing AV + /CD142 + , neutrophil-derived AV + /CD15 + and AV + /CD45 + /CD15 + , and endothelial-derived AV + /CD309 + and CD309 + /CD34 + cMVs were inversely correlated with HbA1c ( r = ‐0.437, r = ‐0.515, r = ‐0.575, r = ‐0.529, r = ‐0.416, and r = ‐0.445, respectively; all p ≤ 0.01), however, only at inclusion. No significant correlations with cardiovascular risk factors were observed.Conclusions Children/adolescents with type 1 diabetes show similar levels of AV + cMVs as healthy controls and limited associations with glucose control. This indicates that our young diabetics on intensive insulin treatment have preserved vascular homeostasis and absence of procoagulant cMVs.