Open AccessmiR-200b/200a/429 Cluster Stimulates Ovarian Cancer Development by Targeting ING5
Author(s) -
Wei Guan,
Huiling Cui,
Ping Huang,
Wan Joo Chun,
Jinwon Lee,
Heasung Kim,
Hua Zou
Publication year - 2020
Publication title -
journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.228
H-Index - 54
eISSN - 1687-8469
pISSN - 1687-8450
DOI - 10.1155/2020/3404059
Subject(s) - ovarian cancer , microrna , cancer research , carcinogenesis , cancer , medicine , malignancy , cell growth , malignant transformation , ovary , oncology , biology , gene , biochemistry , genetics
Ovarian cancer is the second most common gynaecological malignancy, and microRNAs (miRNAs) play important role in the cancer development. Here, we found that the level of miR-200b/200a/429 was significantly increased in serum and tumor tissues of patients with stage-I ovarian cancer. Consistent with these results, we detected increased expression levels of miR-200b/200a/429 in ovarian cancer cell lines compared with the human nontumorigenic ovarian epithelial cell line T80. The overexpression of miR-200b/200a/429 in T80 cells stimulated proliferation and caused their growth in soft agar and tumor formation in nude mice. Furthermore, we determined that miR-200b/200a/429 targets inhibitor of growth family 5 (ING5) and that the overexpression of ING5 can block miR-200b/200a/429-induced T80 cell transformation and tumorigenesis. Our findings suggest that miR-200b/200a/429 may be a useful biomarker for the early detection of ovarian cancer and that miR-200b/200a/429 significantly contributes to ovarian cancer development through ING5.