Open AccessProtective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model
Author(s) -
Liwen Han,
Haotian Kong,
Fasheng Liu,
Xiaobin Li,
Shanshan Zhang,
Xuanming Zhang,
Yong Wu,
Hua Yang,
Aiping Zhang,
Kechun Liu
Publication year - 2019
Publication title -
evidence-based complementary and alternative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.552
H-Index - 90
eISSN - 1741-4288
pISSN - 1741-427X
DOI - 10.1155/2019/8918943
Subject(s) - haematopoiesis , zebrafish , gata1 , erythropoiesis , doxorubicin , pharmacology , apoptosis , hematopoietic growth factor , cancer research , biology , chemotherapy , chemistry , gene , medicine , stem cell , microbiology and biotechnology , anemia , biochemistry
The aim of the study is to explore the protective effect of new gelatin (NG, Xin'ejiao in China) on hematopoietic injury caused by chemotherapy. Zebrafish, at 48 hours post fertilization (hpf), was treated with different chemotherapeutic drugs to establish the zebrafish hematopoietic damage model with reduced thrombocytes and erythrocytes. The protecting effects of NG on the thrombocytes and erythrocytes were observed, respectively, on zebrafish models. Then, the RT-PCR method was used to detect the change of mRNA level of the hematopoiesis-related cytokines scl1 , c-myb , pu.1 , GATA1 , and runx1 genes. The results showed that 50 μ g·mL −1 and 100 μ g·mL −1 NG rescued and increased the thrombocytes numbers induced by vinorelbine (NVB) and chloramphenicol (CHL) and the erythrocytes numbers induced by methotrexate (MTX), doxorubicin (ADM), and mechlorethamine hydrochloride (MH) in zebrafish models. Meanwhile, the mRNA expression of scl1 , c-myb , and GATA1 genes in the NG treatment group was raised compared with the MTX treatment group. Also, the mRNA expression of pu.1 and Runx1 in the NG treatment group was reduced compared with the MTX treatment group. In consequence, traditional Chinese medicine NG showed a certain degree protective effect on hematopoiesis injury induced by chemotherapy in this study, which may depend on the promotion of erythrocytes proliferation and the regulation of the hematopoietic genes level.