Open AccessApoptosis Transcriptional Profile Induced byPorphyromonas gingivalisHmuY
Author(s) -
Paulo Cirino de Carvalho-Filho,
Lília Ferreira de Moura-Costa,
Ana Carla Montino Pimentel,
Mabel Proence Pereira Lopes,
Sibelle A Freitas,
Patrícia Mares de Miranda,
Rosane Dias Costa,
Camila Alexandrina Figueiredo,
Roberto Meyer,
Isaac Suzart GomesFilho,
Teresa Olczak,
Márcia Tosta Xavier,
Soraya Castro Trindade
Publication year - 2019
Publication title -
mediators of inflammation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.37
H-Index - 97
eISSN - 1466-1861
pISSN - 0962-9351
DOI - 10.1155/2019/6758159
Subject(s) - porphyromonas gingivalis , apoptosis , peripheral blood mononuclear cell , gene , gene expression , microbiology and biotechnology , fas ligand , real time polymerase chain reaction , stimulation , biology , downregulation and upregulation , in vitro , immunology , periodontitis , chemistry , medicine , programmed cell death , endocrinology , genetics
This study aimed at evaluating the transcriptional profile of apoptosis-related genes after in vitro stimulation of peripheral blood mononuclear cells (PBMCs) derived from individuals with periodontitis (P) and healthy nonperiodontitis (NP) control subjects with P. gingivalis HmuY protein. PBMCs from the P and NP groups were stimulated with HmuY P. gingivalis protein, and the expression of genes related to apoptosis was assessed by custom real-time polymerase chain reaction array (Custom RT 2 PCR Array). Compared with the NP group, the P group showed low relative levels of apoptosis-related gene expression, downregulated for FAS, FAS ligand, TNFSF10 (TRAIL), BAK1, CASP9, and APAF1 after P. gingivalis HmuY protein stimulation. Furthermore, the P group exhibited low levels of relative gene expression, downregulated for CASP7 when the cells were not stimulated. Our data suggest that P. gingivalis HmuY protein might participate differently in the modulation of the intrinsic and extrinsic apoptosis pathways.