Open AccessHigh-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells
Author(s) -
Sara J. Sidles,
Ying Xiong,
Matthew R. Young,
Amanda C. LaRue
Publication year - 2019
Publication title -
mediators of inflammation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.37
H-Index - 97
eISSN - 1466-1861
pISSN - 0962-9351
DOI - 10.1155/2019/1648614
Subject(s) - adipose tissue , myeloid , biology , t cell , inflammation , medicine , adipose tissue macrophages , endocrinology , immune system , population , cd80 , immunology , white adipose tissue , cytotoxic t cell , cd40 , in vitro , biochemistry , environmental health
Chronic inflammation is evident in the adipose tissue and periphery of patients with obesity, as well as mouse models of obesity. T cell subsets in obese adipose tissue are skewed towards Th1- and Th17-associated phenotypes and their secreted cytokines contribute to obesity-associated inflammation. Our lab recently identified a novel, myeloid-derived CD45 + DDR2 + cell subset that modulates T cell activity. The current study sought to determine how these myeloid-derived CD45 + DDR2 + cells are altered in the adipose tissue and peripheral blood of preobese mice and how this population modulates T cell activity. C57BL/6 mice were fed with a diet high in milkfat (60%·kcal, HFD) ad libitum until a 20% increase in total body weight was reached, and myeloid-derived CD45 + DDR2 + cells and CD4 + T cells in visceral adipose tissue (VAT), mammary gland-associated adipose tissue (MGAT), and peripheral blood (PB) were phenotypically analyzed. Also analyzed was whether mediators from MGAT-primed myeloid-derived CD45 + DDR2 + cells stimulate normal CD4 + T cell cytokine production. A higher percentage of myeloid-derived CD45 + DDR2 + cells expressed the activation markers MHC II and CD80 in both VAT and MGAT of preobese mice. CD4 + T cells were preferentially skewed towards Th1- and Th17-associated phenotypes in the adipose tissue and periphery of preobese mice. In vitro , MGAT from HFD-fed mice triggered myeloid-derived CD45 + DDR2 + cells to induce CD4 + T cell IFN- γ and TNF- α production. Taken together, this study shows that myeloid-derived CD45 + DDR2 + cells express markers of immune activation and suggests that they play an immune modulatory role in the adipose tissue of preobese mice.