Open AccessGAS1Deficient Enhances UPR Activity inSaccharomyces cerevisiae
Author(s) -
Hongjing Cui,
Xingang Cui,
Jing Xia,
Yuan Yuan,
Yaqin Chen,
Yongkun Sun,
Wei Zhao,
Xinguang Liu
Publication year - 2019
Publication title -
biomed research international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 126
eISSN - 2314-6141
pISSN - 2314-6133
DOI - 10.1155/2019/1238581
Subject(s) - tunicamycin , unfolded protein response , saccharomyces cerevisiae , yeast , endoplasmic reticulum , microbiology and biotechnology , biology , cell growth , mutant , biochemistry , gene
Beta-1,3-glucanosyltransferase (Gas1p) plays important roles in cell wall biosynthesis and morphogenesis and has been implicated in DNA damage responses and cell cycle regulation in fungi. Yeast Gas1p has also been reported to participate in endoplasmic reticulum (ER) stress responses. However, the precise roles and molecular mechanisms through which Gas1p affects these responses have yet to be elucidated. In this study, we constructed GAS1- deficient ( gas1Δ ) and GAS1 -overexpressing ( GAS1 OE ) yeast strains and observed that the gas1Δ strain exhibited a decreased proliferation ability and a shorter replicative lifespan (RLS), as well as enhanced activity of the unfolded protein response (UPR) in the absence of stress. However, under the high-tunicamycin-concentration (an ER stress-inducing agent; 1.0 μ g/mL) stress, the gas1Δ yeast cells exhibited an increased proliferation ability compared with the wild-type yeast strain. In addition, our findings demonstrated that IRE1 and HAC1 (two upstream modulators of the UPR) are required for the survival of gas1Δ yeast cells under the tunicamycin stress. On the other hand, we provided evidence that the GAS1 overexpression caused an obvious sensitivity to the low-tunicamycin-concentration (0.25 μ g/mL). Collectively, our results indicate that Gas1p plays an important role in the ageing and ER stress responses in yeast.