Open AccessFood Intake and Core Body Temperature of Pups and Adults in adbMouse Line Deficient in the Long Form of the Leptin Receptor withoutMistyMutation
Author(s) -
Wijang Pralampita Pulong,
Miharu Ushikai,
Akiko Emi,
Masaharu Abe,
Hiroaki Kawaguchi,
Masahisa Horiuchi
Publication year - 2018
Publication title -
journal of diabetes research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.034
H-Index - 50
eISSN - 2314-6753
pISSN - 2314-6745
DOI - 10.1155/2018/9670871
Subject(s) - leptin , food intake , endocrinology , mutation , core (optical fiber) , medicine , leptin receptor , line (geometry) , biology , genetics , obesity , gene , physics , geometry , mathematics , optics
Different involvement of leptin signaling in food intake (FI) and body temperature (BT) in pups and adults has been suggested. However, the leptin receptor (Lepr) long-form-deficient ( db ) mouse line has not been fully examined in pups. In the most available db mouse line, wild-type (WT) mice have a mutation in the dedicator of cytokinesis 7 gene, named misty , which was recently revealed to be involved in neuronal development. Therefore, we established a line of db mice without the misty mutation using natural mating. Adult (8 weeks of age) homozygous db / db mice displayed significantly higher core body weight (BW) and FI and significantly lower core BT than WT mice. However, postnatal (2 weeks of age) db / db mice displayed similar BW and milk intake and significantly lower core BT than WT mice. Correspondingly, adult and postnatal db / db mice exhibited altered mRNA levels of hypothalamic orexigenic and anorexigenic peptide in adults but not in pups. Additionally, db / db mice displayed significantly lower mRNA levels of brown adipose tissue uncoupling protein 1 at both ages. In conclusion, the db mouse line without the misty mutation clearly showed the different involvements of the Lepr long form in FI and BT in pups and adults.