Open AccessHigh-resolution polarization-sensitive optical coherence tomography and optical coherence tomography angiography for zebrafish skin imaging
Author(s) -
Di Yang,
Zhuoqun Yuan,
Zihan Yang,
Muyun Hu,
Yanmei Liang
Publication year - 2021
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s179354582150022x
Subject(s) - zebrafish , optical coherence tomography , microangiography , optical coherence tomography angiography , biomedical engineering , tomography , medicine , pathology , radiology , biology , biochemistry , gene
Zebrafish is an important animal model, which is used to study development, pathology, and genetic research. The zebrafish skin model is widely used in cutaneous research, and angiogenesis is critical for cutaneous wound healing. However, limited by the penetration depth, the available optical methods are difficult to describe the internal skin structure and the connection of blood vessels between the skin and subcutaneous tissue. By a homemade high-resolution polarization-sensitive optical coherence tomography (PS-OCT) system, we imaged the polarization contrast of zebrafish skin and the zebrafish skin vasculature with optical coherence tomography angiography (OCTA). Based on these OCT images, the spatial distribution of the zebrafish skin vasculature was described. Furthermore, we monitored the healing process of zebrafish cutaneous wounds. We think the high-resolution PS-OCT system will be a promising tool in studying cutaneous models of zebrafish.