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Beauveria bassiana  is an insect pathogenic fungus that serves as a model system for exploring the mechanisms of fungal development and host-pathogen interactions. Clinical and experimental studies have indicated that  SND1  is closely correlated with the progression and invasiveness of common cancers as a potential oncogene, but this gene has rarely been studied in fungi. Here, we characterized the contributions of an SND1 ortholog (Tdp1) by constructing a  BbTdp1  deletion strain and a complemented strain of  B. bassiana . Compared with the wild-type (WT) strain, the Δ BbTdp1  mutant lost conidiation capacity (∼87.7%) and blastospore (∼96.3%) yields, increased sensitivity to chemical stress (4.4 to 54.3%) and heat shock (∼44.2%), and decreased virulence following topical application (∼24.7%) and hemocoel injection (∼40.0%). Flow cytometry readings showed smaller sizes of both conidia and blastospores for Δ BbTdp1  mutants. Transcriptomic data revealed 4,094 differentially expressed genes (|log 2  ratio| &gt; 2 and a  q  value of &lt;0.05) between Δ BbTdp1  mutants and the WT strain, which accounted for 41.6% of the total genes, indicating that extreme fluctuation in the global gene expression pattern had occurred. Moreover, deletion of  BbTdp1  led to an abnormal cell cycle with a longer S phase and shorter G 2 /M and G 0 /G 1  phases of blastospores, and enzyme-linked immunosorbent assay confirmed that the level of phosphorylated cyclin-dependent kinase 1 (Cdk1) in the Δ BbTdp1  strain was ∼31.5% lower than in the WT strain. In summary, our study is the first to report that BbTdp1 plays a vital role in regulating conidia and blastospore yields, fungal morphological changes, and pathogenicity in entomopathogenic fungi.   IMPORTANCE  In this study, we used  Beauveria   bassiana  as a biological model to report the role of BbTdp1 in entomopathogenic fungi. Our findings indicated that BbTdp1 contributed significantly to cell development, the cell cycle, and virulence in  B. bassiana . In addition, deletion of  BbTdp1  led to drastic fluctuations in the transcriptional profile. BbTdp1 can be developed as a novel target for  B. bassiana  development and pathogenicity, which also provides a framework for the study of Tdp1 in other fungi.

microbiology spectrum

The Tudor Domain-Containing Protein BbTdp1 Contributes to Fungal Cell Development, the Cell Cycle, Virulence, and Transcriptional Regulation in the Insect Pathogenic Fungus Beauveria bassiana