English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We previously reported that a human immunodeficiency virus type 1 (HIV-1) envelope (Env) mutant with the whole cytoplasmic domain deleted, denoted mutant TC, is able to dominantly interfere with wild-type (wt) virus infectivity. In the present study, the feasibility of developing a dominant negative mutant-based genetic anti-HIV strategy targeting the gp41 cytoplasmic domain was investigated. Mutants TC and 427,TC, a TC derivative with a Trp-to-Ser substitution introduced into residue 427 in the CD4-binding site, and a series of mutants with deletions in the cytoplasmic domain, effectivelytrans -dominantly interfered with wt Env-mediated viral infectivity, as demonstrated by anenv trans -complementation assay. The syncytium formation-defective 427,TC double mutant not only inhibited heterologous LAV and ELI Env-mediated viral infectivity but also interfered with syncytium formation and infectivity mediated by the Env proteins of the two primary isolates 92BR and 92US. Stable HeLa-CD4-LTR-β-gal clones that harbored Tat-controlled expression cassettes encoding the control ΔKS, which had a deletion in theenv gene, wt, or mutantenv gene were generated. Viral transmission mediated by laboratory-adapted T-cell-tropic HXB2 and NL4-3 viruses was greatly reduced in the TC and 427,TC transfectants compared to that observed in the control ΔKS and wt transfectants. Viral replication caused by HXB2 and NL4-3 viruses and by macrophage-tropic ConB and ADA-GG viruses was delayed or reduced in human CD4+ T cells transfected with the 427,TCenv construct compared to that observed in cells transfected with the control ΔKS or TCenv construct. The lack of significant interference by TC mutant was due neither to the lack of TCenv gene integration into host DNA nor to the lack of TC Env expression upon Tat induction. These results indicate that this 427,TC Env double mutant has a role in the development oftrans -dominant mutant-based genetic anti-HIV strategies.

journal of virology

<i>trans</i>-Dominant Interference with Human Immunodeficiency Virus Type 1 Replication and Transmission in CD4<sup>+</sup>Cells by an Envelope Double Mutant

trans-Dominant Interference with Human Immunodeficiency Virus Type 1 Replication and Transmission in CD4+Cells by an Envelope Double Mutant

trans-Dominant Interference with Human Immunodeficiency Virus Type 1 Replication and Transmission in CD4⁺Cells by an Envelope Double Mutant