Open AccessCharacterization of human endogenous retroviral envelope RNA transcripts
Author(s) -
Arnold B. Rabson,
Yasutaro Hamagishi,
P E Steele,
Mark L. Tykocinski,
M A Martin
Publication year - 1985
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.56.1.176-182.1985
Subject(s) - biology , complementary dna , polyadenylation , rna , microbiology and biotechnology , endogenous retrovirus , northern blot , gene , coding region , cloning (programming) , exon , genetics , virology , genome , computer science , programming language
We characterized the structure of human endogenous retroviral env RNA transcripts by Northern blot hybridization and cDNA cloning. Polyadenylated 3.0- and 1.7-kilobase env RNAs can be identified in placenta, colon carcinoma, and breast carcinoma cells. We have obtained partial cDNA clones of both size classes of RNAs. Both env RNAs contained putative gp70 coding sequences; the 1.7-kilobase species, however, lacked sequences in the 3' env region which could specify a p15E analog. Both cDNA clones contained in-frame termination codons; thus, neither could encode full-length env proteins.
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