Open AccessOrganization of viral genome in a T antigen-negative hamster tumor induced by human papovavirus BK
Author(s) -
Yoshiaki Yogo,
Akemi Furuno,
A Nozawa,
Seijiro Uchida
Publication year - 1981
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.38.2.556-563.1981
Subject(s) - papovavirus , biology , bk virus , virology , microbiology and biotechnology , antigen , southern blot , genome , hamster , nucleic acid thermodynamics , dna , virus , genetics , gene , kidney transplantation , kidney , base sequence
We analyzed by blot hybridization the state and structure of the viral DNA in an exceptional BK virus-induced hamster tumor (choroid plexus papilloma Vn-324) that contains about one copy of the BK virus genome per cell, but no intranuclear T antigen as assayed by indirect immunofluorescence. The BK viral DNA was found to be integrated into cellular DNA at a site in the middle of the early region of the viral genome (between 0.32 and 0.41 map units). The structure of the inserted viral DNA shows that it cannot encode a full-size large T antigen, but may encode small T antigen and an N-terminal portion of large T antigen.
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