Open AccessPichinde virus L and S RNAs contain unique sequences
Author(s) -
WaiChoi Leung,
Arlene I. Ramsingh,
Kenneth Dimock,
William E. Rawls,
J Petrovich,
Maria FKL Leung
Publication year - 1981
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.37.1.48-54.1981
Subject(s) - complementary dna , biology , rna , primer (cosmetics) , microbiology and biotechnology , dna , arenavirus , nucleic acid thermodynamics , virus , transcription (linguistics) , virology , gene , in vitro , lymphocytic choriomeningitis , genetics , cytotoxic t cell , chemistry , organic chemistry , philosophy , linguistics
Using oligodeoxyribonucleotides produced by limited DNase I digestion of calf thymus DNA as a primer, we synthesized complementary DNA (cDNA) from the L and the S RNAs of Pichinde virus. The reaction conditions for in vitro cDNA synthesis were optimized to allow transcription of about 90% of either L or S RNA. No significant hybridization was observed when the L cDNA was hybridized to the S RNA, or when the S cDNA was hybridized to the L RNA. The results indicate that the L and S RNAs of Pichinde virus contain unique nucleotide sequences.
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