Open AccessReplication process of the parvovirus H-1. IX. Physical mapping studies of the H-1 genome
Author(s) -
S L Rhode
Publication year - 1977
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.22.2.446-458.1977
Subject(s) - biology , hpaii , haeiii , ecori , dna replication , dna , restriction enzyme , microbiology and biotechnology , inverted repeat , cleavage (geology) , hindiii , genetics , genome , gene , polymerase chain reaction , fracture (geology) , dna methylation , restriction fragment length polymorphism , paleontology , gene expression
The cleavage map of H-1 replicative-form DNA to the bacterial restriction endonuclease EcoRI, HaeII, HaeIII, HindII, HindIII, and HpaII has been determined. The 5'-phosphoryl end of the viral strand is on the right end of the molecule at or near the replication origin. Evidence is presented for the presence of inverted self-complementary sequences at the right end that differ from those at the left end. These sequences allow a foldback of the DNA after denaturation, and a minority of the native replicative-form DNA has the foldback configuration. The possible role of these structures in H-1 DNA synthesis is discussed.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom