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Understanding the concepts of molecular biology and then applying those  concepts to laboratory experiments can be challenging to entry-level  students. In order to facilitate the topics of restriction enzyme  digestion and the generation of compatible ends in the process of gene  cloning, an in-class activity was designed. This restriction enzyme  digestion exercise, designed for an introductory undergraduate course in  genetics, molecular biology and molecular diagnostics, can be utilized  in either a lecture or laboratory setting. Students are provided with  information on enzyme discovery and origin, sticky, blunt and compatible  ends, base-cutters, isoschizomers and isocaudomers. Students then review the components required for restriction enzyme  digestion setup, such as DNA concentration, buffer volume and  compatibility and multi-enzyme digestions. Upon completion of the theory review, students participate in this  classroom activity where scissors and paper replace restriction enzymes  and DNA, providing a visual learning experience

Restriction Enzyme Digestion Exercise – An In-class Activity