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Of the several microsporidia that infect humans,Enterocytozoon bieneusi is known to cause a gastrointestinal disease whereasEncephalitozoon intestinalis causes both a disseminated and an intestinal disease. Although several different staining techniques, including the chromotrope technique and its modifications, Uvitex 2B, and the quick-hot Gram-chromotrope procedure, detect microsporidian spores in fecal smears and other clinical samples, they do not identify the species of microsporidia. A need for an easily performed test therefore exists. We reevaluated 120 stool samples that had been found positive for microsporidia previously, using the quick-hot Gram-chromotrope technique, and segregated them into two groups on the basis of spore size. We also screened the smears by immunofluorescence microscopy, using a polyclonal rabbit anti-E. intestinalis serum at a dilution of 1:400. Spores in 29 (24.1%) of the 120 samples fluoresced brightly, indicating that they wereE. intestinalis spores. No intense background or cross-reactivity with bacteria, yeasts, or other structures in the stool samples was seen. Additionally, the numbers of spores that fluoresced in seven of these samples were substantially smaller than the numbers of spores that were present in the stained smears, indicating that these samples were probably derived from patients with mixed infections ofEnterocytozoon bieneusi andE. intestinalis . Because a 1:400 dilution of this serum does not react with culture-grownEncephalitozoon hellem ,Encephalitozoon cuniculi , orVittaforma corneae or withEnterocytozoon bieneusi spores in feces, we concluded that an immunofluorescence test using this serum is a good alternative for the specific identification ofE. intestinalis infections.

journal of clinical microbiology

Detection by an Immunofluorescence Test of <i>Encephalitozoon intestinalis</i> Spores in Routinely Formalin-Fixed Stool Samples Stored at Room Temperature

Detection by an Immunofluorescence Test of Encephalitozoon intestinalis Spores in Routinely Formalin-Fixed Stool Samples Stored at Room Temperature