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Staphylococci (n = 130) of bovine origin representing 14 species were evaluated. Agreements of Vitek and API systems with conventional methods were 44.6 and 80.8%, respectively. The poor performance of the Vitek system was attributed primarily to inability to identify S. chromogenes. Incorporation of additional veterinary strains into the Vitek data base is needed to increase accuracy.

Comparison of Vitek Gram-Positive Identification system with API Staph-Trac system for species identification of staphylococci of bovine origin