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A new commercial system (API 20 Strep) for the species identification of streptococci associated with bovine mastitis was compared with the conventional biochemical methods. A total of 84 strains, including Streptococcus agalactiae (13), S. dysgalactiae (16), S. uberis (24), S. faecalis (12), S. faecium (5), and S. bovis (14) were tested. Using profile index and table data issued by the manufacturer, we identified 71.4% of strains by the API 20 Strep system. Our results indicate that improvement of the identification key is needed for accurate identification of streptococci isolated from bovine mastitis.

Evaluation of the API 20 Strep system for species identification of streptococci isolated from bovine mastitis