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An upper limit for β-galactosidase transfer between mating F+ and F− Escherichia coli has been determined by a new technique which relies on selective lysis of the donor strain by heat induction of a thermo-inducible strain of λ, accompanied by chymotryptic digestion of the released β-galactosidase. No significant transfer of β-galactosidase during mating between F+ and F− cells has been observed: 0.05 ± 0.05% of the enzyme originally present in the male cells is found in the female cells after 1 hr of mating at 37 C.

An Upper Limit on β-Galactosidase Transfer in Bacterial Conjugation