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Johnson , E. M. (Walter Reed Army Institute of Research, Washington, D.C.),Barbara Krauskopf, and L. S. Baron . Genetic analysis of theViA-his chromosomal region inSalmonella . J. Bacteriol.92: 1457–1463. 1966.—The relative chromosomal location of theViA determinant, a gene required for Vi antigen expression inSalmonella typhosa (and present also inS. typhimurium ), was examined inS. typhimurium ×S. typhosa matings. The position of this gene was determined with respect to the histidine (his ) and methionine (metG ) biosynthesis markers, and to the genetic determinants of somatic antigens 5 (O-5 ) and 4 (O-4 ) ofS. typhimurium . The gene order established by analyses of the hybrid classes resulting from the genetic crosses wasViA–O-5–metG–O-4 (his ). This order suggests that neitherViA norO-5 is a member of the complex of functionally related structural genes which constitute theO-4 locus. It allows for the possibility, however, of a functional relationship between the genes of theViA andO-5 loci.

Genetic Analysis of theViA-hisChromosomal Region inSalmonella