Nitrite Reductase of Escherichia coli Specific for Reduced Nicotinamide Adenine Dinucleotide

Kemp, John D. (University of California, Los Angeles),and Daniel E. Atkinson . Nitrite reductase ofEscherichia coli specific for reduced nicotinamide adenine dinucleotide. J. Bacteriol.92: 628–634. 1966.—A nitrite reductase specific for reduced nicotinamide adenine dinucleotide (NADH2 ) appears to be responsible for in vivo nitrite reduction byEscherichia coli strain Bn. In extracts, the reduction product is ammonium, and the ratio of NADH2 oxidized to nitrite reduced or to ammonium produced is 3. The Michaelis constant for nitrite is 10 μm . The enzyme is induced by nitrite, and the ability of intact cells to reduce nitrite parallels the level of NADH2 -specific nitrite reductase activity demonstrable in cell-free preparations. Crude extracts of strain Bn will also reduce hydroxylamine, but not nitrate or sulfite, at the expense of NADH2 . Kinetic observations indicate that hydroxylamine and nitrite may both be reduced at the same active site. The high apparent Michaelis constant for hydroxylamine (1.5 mm ), however, seems to exclude hydroxylamine as an intermediate in nitrite reduction. In vitro activity is enhanced by preincubation with nitrite, and decreased by preincubation with NADH2 .