Purification and Characterization of Clostridium botulinum Type B Toxin

Gerwing, Julia (The University of British Columbia, Vancouver, B.C., Canada),Claude E. Dolman, David V. Kason, and Jack H. Tremaine . Purification and characterization ofClostridium botulinum type B toxin. J. Bacteriol.91: 484–487. 1966.—A toxic component of low molecular weight has been isolated from a type B strain ofClostridium botulinum by methods involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose atp H 5.6. The material thus isolated was shown to be monophoretic and monodisperse in the ultracentrifuge. End-group analysis indicated the presence of a single N-terminal amino acid residue, which was identified as arginine. On the basis of biophysical studies and amino acid analyses, a molecular weight between 9,000 and 10,000 was calculated.