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Ensign , J. C. (University of Illinois, Urbana),and R. S. Wolfe . Lysis of bacterial cell walls by an enzyme isolated from a myxobacter. J. Bacteriol.90: 395–402. 1965.—An exoenzyme which lyses intact cells, heat-killed cells, and cell walls ofArthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain AL-1). The lytic enzyme was produced during growth of the organism in a number of complex media, the maximal amount of enzyme being produced in yeast extract broth. The purified enzyme lysed at different rates a number of gram-positive bacteria. With the exception ofRhodospirillum rubrum, Spirillum itersonii , andS. serpens , the gram-negative bacteria tested were not attacked.

Lysis of Bacterial Cell Walls by an Enzyme Isolated from a Myxobacter