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Hardy, Paul H., Jr . (The Johns Hopkins University School of Medicine, Baltimore, Md.),Young C. Lee, and E. Ellen Nell . Use of a bacterial culture filtrate as an aid to the isolation and growth of anaerobic spirochetes. J. Bacteriol.87: 1521–1525. 1964.—A previously described procedure that permits colonial growth of laboratory-adapted anaerobic spirochetes on surface streaked media was applied to the isolation of new spirochetal strains from human oral microflora. Primary isolates were readily cultivable as colonies, but they were found to be nutritionally dependent upon other microorganisms with which they are normally associated in vivo. Spirochetal growth was first noted around colonies of oral bacteria; pure cultures were then grown on media fortified with the culture filtrate of one such organism—a microaerophilic diphtheroid.

USE OF A BACTERIAL CULTURE FILTRATE AS AN AID TO THE ISOLATION AND GROWTH OF ANAEROBIC SPIROCHETES