FRUCTOSE-6-PHOSPHATE REDUCTASE FROM SALMONELLA GALLINARUM

Zancan, Glaci T. (Universidade do Paraná, Curitiba, Paraná, Brazil),and Metry Bacila . Fructose-6-phosphate reductase fromSalmonella gallinarum . J. Bacteriol.87: 614–618. 1964.—A fructose-6-phosphate reductase present in cell-free extracts ofSalmonella gallinarum was purified approximately 42 times. The optimal pH for this enzyme is 8.0. The enzyme is specific for fructose-6-phosphate and reduced nicotinamide adenine dinucleotide (NADH). The dissociation constants are 1.78 × 10−4 m for fructose-6-phosphate and 8.3 × 10−5 m for NADH. The Q10 , reaction order, and equilibrium constant were determined. The enzyme is sensitive top -chloromercuribenzoic acid, but not too -iodosobenzoic acid nor toN -ethylmaleimide.