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Toohey , J. I. (University of California, Berkeley),and H. A. Barker . Factors affecting the formation of cobamide coenzymes inClostridium tetanomorphum . J. Bacteriol.87: 504–509. 1964.—Tests were carried out to determine the optimal culture conditions for the production of cobamide coenzymes inClostridium tetanomorphum strain H1. A method is described for carrying out coenzyme determinations on the cells from 10-ml cultures of the bacterium. In a basal medium containing magnesium sulfate, ferrous sulfate, manganese sulfate, sodium molybdate, calcium chloride, and potassium phosphate, the optimal concentration of monosodium glutamate was 0.1m and of yeast extract was 3 g per liter. Addition of glucose at a concentration of 0.05m was found to double the yield of cells and to increase tenfold the specific coenzyme yield. Addition of cobaltous chloride (2 × 10−5 m ) also increased coenzyme production. Addition of benzimidazole caused an apparent increase in coenzyme production by causing the synthesis of the highly active benzimidazole analogue. Addition of methionine (5 × 10−6 m ) appeared to inhibit coenzyme production.

FACTORS AFFECTING THE FORMATION OF COBAMIDE COENZYMES IN CLOSTRIDIUM TETANOMORPHUM