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We identified Mg2+ -responsive promoters of thephoPQ ,mgtA , andmgrB genes ofEscherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repeat in their corresponding promoter regions.

journal of bacteriology

Molecular Characterization of the PhoP-PhoQ Two-Component System in <i>Escherichia coli</i> K-12: Identification of Extracellular Mg <sup>2+</sup> -Responsive Promoters

Molecular Characterization of the PhoP-PhoQ Two-Component System in Escherichia coli K-12: Identification of Extracellular Mg 2+ -Responsive Promoters

Molecular Characterization of the PhoP-PhoQ Two-Component System in Escherichia coli K-12: Identification of Extracellular Mg ²⁺ -Responsive Promoters