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We purified from the culture supernatant ofAlteromonas sp. strain O-7 and characterized a transglycosylating enzyme which synthesized β-(1→6)-(GlcNAc)2 , 2-acetamido-6-O -(2-acetamido-2-deoxy-β-d -glucopyranosyl)-2-deoxyglucopyranose from β-(1→4)-(GlcNAc)2 . The gene encoding a novel transglycosylating enzyme was cloned intoEscherichia coli , and its nucleotide sequence was determined. The molecular mass of the deduced amino acid sequence of the mature protein was determined to be 99,560 Da which corresponds very closely with the molecular mass of the cloned enzyme determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular mass of the cloned enzyme was much larger than that of enzyme (70 kDa) purified from the supernatant of this strain. These results suggest that the native enzyme was the result of partial proteolysis occurring in the N-terminal region. The enzyme showed significant sequence homology with several bacterial β-N -acetylhexosaminidases which belong to family 20 glycosyl hydrolases. However, this novel enzyme differs from all reported β-N -acetylhexosaminidases in its substrate specificity. To clarify the role of the enzyme in the chitinolytic system of the strain, the effect of β-(1→6)-(GlcNAc)2 on the induction of chitinase was investigated. β-(1→6)-(GlcNAc)2 induced a level of production of chitinase similar to that induced by the medium containing chitin. On the other hand, GlcNAc, (GlcNAc)2 , and (GlcNAc)3 conversely repressed the production of chitinase to below the basal level of chitinase activity produced constitutively in medium without a carbon source.

journal of bacteriology

Molecular Analysis of the Gene Encoding a Novel Transglycosylative Enzyme from <i>Alteromonas</i> sp. Strain O-7 and Its Physiological Role in the Chitinolytic System

Molecular Analysis of the Gene Encoding a Novel Transglycosylative Enzyme from Alteromonas sp. Strain O-7 and Its Physiological Role in the Chitinolytic System