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All mycobacteria studied to date have an rRNA operon, designatedrrnA , located downstream from a single copy of themurA gene, which encodes an enzyme (EC2.5.1.7 ) important for peptidoglycan synthesis. TherrnA operon has a promoter, P1(A), located within the coding region ofmurA , near the 3′ end. Samples of RNA were isolated fromMycobacterium tuberculosis at different stages of the growth cycle and fromMycobacterium smegmatis grown under different conditions. RNase protection assays were used to investigate transcripts of bothmurA andrrnA . Transcription ofmurA was found to continue into the 16S rRNA gene, as ifmurA andrrnA form a hybrid (protein coding-rRNA coding) operon. During the growth ofM. tuberculosis , the hybrid operon contributed approximately 2% to total pre-rRNA. Analysis ofM. smegmatis RNA revealed that the level ofmurA RNA depended on the growth rate and that the patterns of expression during the growth cycle were different formurA andrrnA .M. smegmatis has a second rRNA operon,rrnB , located downstream from a single copy of thetyrS gene, encoding tyrosyl-tRNA synthetase. Transcription oftyrS was found to continue into the 16S rRNA generrnB . The hybridtyrS-rrnB operon contributed 0.2 to 0.6% torrnB transcripts. The pattern oftyrS expression during the growth cycle matched the pattern ofrrnB expression, reflecting the essential role of TyrS and rRNA in protein biosynthesis.

journal of bacteriology

Effects of Growth Conditions on Expression of Mycobacterial <i>murA</i> and <i>tyrS</i> Genes and Contributions of Their Transcripts to Precursor rRNA Synthesis

Effects of Growth Conditions on Expression of Mycobacterial murA and tyrS Genes and Contributions of Their Transcripts to Precursor rRNA Synthesis