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TheKlebsiella pneumoniae nitrogen fixation (nif ) mRNAs are unusually stable, with half-lives of 20 to 30 min under conditions favorable to nitrogen fixation (limiting nitrogen, anaerobiosis, temperatures of 30°C). Addition of O2 or fixed nitrogen or temperature increases to 37°C or more result in the dramatic destabilization of thenif mRNAs, decreasing the half-lives by a factor of 3 to 5. A plasmid expression system, independent ofnif transcriptional regulation, was used to definecis determinants required for the regulated stability of the 5.2-kbnifHDKTY mRNA and to test the model suggested by earlier work that NifA is required intrans to stabilizenif mRNA undernif -derepressing conditions. O2 regulation ofnifHDKTY mRNA stability is impaired in a plasmid containing a deletion of a 499-bp region ofnifH , indicating that a site(s) required for the O2 -regulated stability of the mRNA is located within this region. The simple model suggested from earlier work that NifA is required for stabilizingnif mRNA under conditions favorable for nitrogen fixation was disproved, and in its place, a more complicated model involving the sensing of nitrogenase activity as a component of the system regulating mRNA stability is proposed. Analysis ofnifY mutants and overexpression suggests a possible involvement of the protein in this sensing process.

journal of bacteriology

Importance of <i>cis</i> Determinants and Nitrogenase Activity in Regulated Stability of the <i>Klebsiella pneumoniae</i> Nitrogenase Structural Gene mRNA

Importance of cis Determinants and Nitrogenase Activity in Regulated Stability of the Klebsiella pneumoniae Nitrogenase Structural Gene mRNA