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A cluster of at least 14 genes, each concerned with some aspect of cell envelope growth, morphogenesis, or function, is located at 2 min on the genetic map of Escherichia coli. We located the envA cell division gene and its promoter within the cluster and determined the direction of transcription of the gene by constructing fusions between its promoter and the galK coding sequence. In addition, we identified the promoter of a possible new gene lying between envA and the secA gene. We also present evidence from gene fusion studies which shows the direction of transcription of the ftsZ(sulB) division gene. The direction of transcription is the same for all three promoters and is the same as that of all other cluster genes for which this is known. We discuss the significance of this observation, together with the fact that every gene examined in sufficient detail within the cluster appears to have its own promoter and to be able to be expressed from isolated cloned fragments. Using a novel variable-copy plasmid vector, we demonstrate that the DNA fragment containing the envA gene is not stably maintained in multiple copies. The construction of two independent, nonoverlapping deletions allows us to conclude that the envA product itself is responsible for this effect.

Transcriptional organization within an Escherichia coli cell division gene cluster: direction of transcription of the cell separation gene envA