Open AccessPlasmid containing a DNA ligase gene from Haemophilus influenzae
Author(s) -
David McCarthy,
K Griffin,
Jane K. Setlow
Publication year - 1984
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.158.2.730-732.1984
Subject(s) - biology , haemophilus influenzae , dna ligase , plasmid , shuttle vector , mutant , ligase chain reaction , gene , microbiology and biotechnology , haemophilus , escherichia coli , dna , vector (molecular biology) , genetics , bacteria , recombinant dna , polymerase chain reaction , antibiotics , multiplex polymerase chain reaction
A ligase gene from Haemophilus influenzae was cloned into the shuttle vector pDM2 . Although the plasmid did not affect X-ray sensitivity, it caused an increase in UV sensitivity of the wild-type but not excision-defective H. influenzae and a decrease in UV sensitivity of the rec-1 mutant.
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