Open AccessSubcloning of the cloacin DF13/aerobactin receptor protein and identification of a pColV-K30-determined polypeptide involved in ferric-aerobactin uptake
Author(s) -
W Krone,
Joen Luirink,
Gregory M. Koningstein,
Bauke Oudega,
F K de Graaf
Publication year - 1983
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.156.2.945-948.1983
Subject(s) - aerobactin , subcloning , biology , plasmid , escherichia coli , ferric , biochemistry , enterobacteriaceae , microbiology and biotechnology , gene , chemistry , organic chemistry
A plasmid containing a pColV-K30 fragment that encoded only for the cloacin DF13/aerobactin receptor protein was constructed. Escherichia coli cells harboring this plasmid were sensitive to cloacin DF13 but were unable to take up ferric-aerobactin. Another pColV-K30-determined polypeptide (molecular weight, 50,000), localized in the membrane fraction, was essential for the uptake of ferric-aerobactin.
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