Open AccessCloning of sporulation genes spo0A and spo0C of Bacillus subtilis onto rho 11 temperate bacteriophage
Author(s) -
T. Ikeuchi,
Jun Kudoh,
K. Kurahashi
Publication year - 1983
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.154.2.988-991.1983
Subject(s) - biology , bacillus subtilis , bacteriophage , hindiii , cloning (programming) , temperateness , gene , transformation (genetics) , dna , microbiology and biotechnology , genetics , molecular cloning , plasmid , bacteria , escherichia coli , gene expression , computer science , programming language
A HindIII fragment harboring the intact spo0C gene of Bacillus subtilis was cloned with rho 11 temperate bacteriophage as a vector. Transformation experiments with the DNA from rho 11 dspo0C+ specialized transducing phage showed that the spo0C gene resides on a 5.3-megadalton fragment generated by HindIII digestion. The 5.3-megadalton fragment also contains the intact spo0A gene, but not spoIIIA, spoIIIB, or spoIVB.
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