Open AccessIsolation of mycoplasma membranes by dicyclohexylcarbodiimide-induced lysis
Author(s) -
Mitchell H. Shirvan,
Shlomo Rottem,
Zvi Neʼeman,
Robert Bittman
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.149.3.1124-1128.1982
Subject(s) - lysis , mycoplasma gallisepticum , biology , membrane , cytolysis , isolation (microbiology) , mycoplasma , atpase , biochemistry , cell membrane , microbiology and biotechnology , enzyme , in vitro , cytotoxicity
A simple procedure was devised to prepared membranes from Mycoplasma gallisepticum cells. The cells were lysed in an isosmotic NaCl solution by dicyclohexylcarbodiimide, which blocks ATPase activity and interferes with the regulation of cell volume. The procedure can be used to isolate membranes of other osmotically resistant mycoplasmas.
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