Open AccessPenicillin selection of Escherichia coli deoxyribonucleic acid repair mutants
Author(s) -
Aziz Sancar,
Claud S. Rupert
Publication year - 1979
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.138.3.779-782.1979
Subject(s) - biology , escherichia coli , photolyase , mutant , microbiology and biotechnology , dna repair , penicillin , dna , mutation , ultraviolet light , ultraviolet radiation , genetics , antibiotics , gene , chemistry , radiochemistry , photochemistry
A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.
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