Open AccessIn vivo titration of araC protein
Author(s) -
D M Haggerty,
Max P. Oeschger,
Robert Schleif
Publication year - 1978
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.135.3.775-781.1978
Subject(s) - operon , biology , escherichia coli , lac operon , mutation , l arabinose operon , in vivo , microbiology and biotechnology , genetics , gene
The requirement for araC protein in the induction of the araBAD operon was investigated. Strains of Escherichia coli carrying an araC(Am) mutation and temperature-sensitive amber suppressors were used to vary the intracellular level of araC protein. The levels of araC protein studied ranged from 0.007 to 1.8 times the normal amount. The results indicate that the normal level of araC protein is just sufficient to provide maximal expression of the araBAD operon.
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