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Starting with a previously isolated F′his episome and φ80 dhis immλ cI857transducing phage, we have constructed recombinant elements bearing previously isolatedhis mutations fromSalmonella typhimurium . These phages were constructed as sources of deoxyribonucleic acid for in vitro biochemical experiments on gene regulation. The manipulation of genes betweenS. typhimurium andEscherichia coli described here may be useful in studying otherS. typhimurium operons.

Construction of φ80 dhisCarryingSalmonella typhimuriumHistidine Operon Mutations