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Clumping of bacteria containing R factors derepressed for pilus synthesis interfered with the separation of cells and minicells. Mucoid derivatives of these bacteria provided the means of preventing clumping and allowing a good separation. This method may be generally useful in dealing with autoagglutination of bacterial cultures.

Use of Mucoid Bacterial Mutants to Circumvent Clumping of Cells and Minicells Containing R Plasmids Derepressed for Pilus Synthesis