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A mutation that causes a temperature-sensitive RecA− phenotype was identified in a derivative of a PolA− strain that failed to grow at high temperature. The mutant allele (recA200 ) was shown to be linked tocysC , conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec− phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferredrecA − . Double mutants that carried bothrecA200 andpolA were examined for ability to grow and synthesize DNA at restrictive temperatures.

Isolation and Characterization of an Escherichia coli K-12 Mutant with a Temperature-Sensitive RecA − Phenotype

Isolation and Characterization of an Escherichia coli K-12 Mutant with a Temperature-Sensitive RecA ⁻ Phenotype