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A mutant strain ofEscherichia coli unable to carry out the first specific reaction of ubiquinone biosynthesis, that is the conversion of chorismate into 4-hydroxybenzoate, has been isolated. The gene concerned maps at about minute 79 on theE. coli chromosome and has been designatedubiC . This gene is probably the structural gene for chorismate lyase since cell extracts from a transductant strain carrying theubiC437 mutant allele are unable to convert chorismate into 4-hydroxybenzoate and growing cells of the mutant do not form appreciable quantities of ubiquinone unless 4-hydroxybenzoate is added to the growth medium.

journal of bacteriology

Biosynthesis of Ubiquinone in <i>Escherichia coli</i> K-12: Biochemical and Genetic Characterization of a Mutant Unable to Convert Chorismate into 4-Hydroxybenzoate

Biosynthesis of Ubiquinone in Escherichia coli K-12: Biochemical and Genetic Characterization of a Mutant Unable to Convert Chorismate into 4-Hydroxybenzoate