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The effect of thymine deprivation on the integrity of phage λ, sex factor, and chromosomal deoxyribonucleic acid (DNA) inEscherichia coli CR34 (λind − ) was examined by sedimenting cell lysates through alkaline sucrose gradients. Both sex factor and chromosomal DNAs showed evidence of being degraded during the starvation period. In contrast, no loss of closed circular λ DNA was observed.

journal of bacteriology

Influence of Thymine Starvation on the Integrity of Episomal and Chromosomal Deoxyribonucleic Acids in Escherichia coli CR34 (λ ind − )