Open AccessMutant of Bacillus subtilis with a Temperature-Sensitive Autolytic Amidase
Author(s) -
David P. Fan,
Mary M. Beckman
Publication year - 1973
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.114.2.798-803.1973
Subject(s) - autolysin , amidase , biology , mutant , autolysis (biology) , bacillus subtilis , biochemistry , enzyme , microbiology and biotechnology , gene , bacteria , genetics
A procedure incorporating a petri plate assay for autolysins has been developed for the systematic isolation of mutants with a temperature-sensitive autolytic amidase. We suggest maa as the name for the gene for this enzyme. One such mutant has been characterized as having a heat-sensitive amidase in a variety of studies with both crude autolysin preparations and purified amidase solutions.
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