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Hybrids betweenEscherichia coli K-12 andSalmonella typhosa which conserved a continuous K-12 chromosomal diploid segment extending frompro throughara to thestrA locus were sensitive to plaque formation by wild-type λ. These partially diploidS. typhosa hybrids could be lysogenized with λ and subsequently induced to produce infectious phage particles. When the K-12 genes were segregated from a lysogenicS. typhosa hybrid, phage-productive ability was no longer detectable due to loss of a genetic region necessary for vegetative replication of λ. However, λ prophage was shown to persist in a quiescent state in theS. typhosa hybrid segregant with phage-productive ability being reactivated after replacement of the essential K-12 λ replication region. Low-frequency transduction and high-frequency transduction lysates containing thegal + genes ofS. typhosa were prepared by induction of λ-lysogenicS. typhosa hybrids indicating that theatt λ site is chromosomally located inS. typhosa in close proximity to thegal locus as inE. coli K-12. After propagation inS. typhosa hybrids, λ was subject to restriction byE. coli K-12 recipients, thus establishing thatS. typhosa does not perform the K-12 modification of λ deoxyribonucleic acid. Hybrids ofS. typhosa , however, did not restrict λ grown previously onE. coli K-12. The K-12 genetic region required for λ phage production inS. typhosa was located within min 66 to min 72 on the genetic map of theE. coli chromosome. Transfer of an F-merogenote encompassing the 66 to 72 minE. coli chromosomal region to λ-insensitiveS. typhosa hybrids enabled them to replicate wild-type λ. The λ-insensitiveS. typhosa hybrid, WR4255, which blocks λ replication, can be mutagenized to yield mutant strains sensitive to λvir and λimm 434. These WR4255 mutants remained insensitive to plaque formation by wild-type λ.

Lytic Replication of Coliphage Lambda in Salmonella typhosa Hybrids