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When heat-activated spores ofBacillus cereus T (thy − ) were germinated and grown in medium containing3 H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from3 H-thymidine into DNA only. This was accomplished by labeling the cells with3 H-thymidine in the presence of 2 mg of 2-deoxyadenosine per ml, 250 μg each of uracil, cytosine, and guanosine per ml, and 500 μg of adenosine per ml. Under these conditions, 97% of the radioactivity incorporated into cold trichloroacetic acid-insoluble material was associated with DNA only. In the absence of these compounds, DNA contained only 72% of the total radioactivity incorporated into cold acid-insoluble material.

journal of bacteriology

Method for Restricting Incorporation of Radioactivity from <sup>3</sup> H-Thymidine into Deoxyribonucleic Acid Only During Outgrowth of Spores of <i>Bacillus cereus</i> T

Method for Restricting Incorporation of Radioactivity from 3 H-Thymidine into Deoxyribonucleic Acid Only During Outgrowth of Spores of Bacillus cereus T

Method for Restricting Incorporation of Radioactivity from ³ H-Thymidine into Deoxyribonucleic Acid Only During Outgrowth of Spores of Bacillus cereus T