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The arabinogalactan isolated from the cell wall ofMycobacterium bovis reacts to form a precipitate with concanavalin A, the phytohemagglutinin of the jack bean. A typical precipitin-like curve is obtained when increasing amounts of the polysaccharide are added to a constant quantity of concanavalin A. Inhibitor studies suggest that concanavalin A reacts with the arabinogalactan by interacting with the C-2, C-3, and C-5 hydroxyl groups of the α-d -arabinofuranosyl residues situated at the chain ends of this polysaccharide.

Interaction of Concanavalin A with an Arabinogalactan from the Cell Wall ofMycobacterium bovis