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Unlike five other strains ofMucor rouxii previously studied, certain nutritional factors must be present for rapid growth and completely yeastlike development ofM. rouxii (National Regional Research Laboratory 1894) under CO2 ; high CO2 tensions markedly inhibit growth of this strain. Addition of yeast extract, peptone, or enzymatically hydrolyzed casein in substrate amounts to a basal medium (containing acid-hydrolyzed casein) completely relieved CO2 inhibition of growth and permitted yeastlike development. The “CO2 growth factor” activity of these supplements proved to be dialyzable and acid labile. These findings, together with the results of gel filtration and amino acid analysis, suggested that CO2 growth factor activity can be attributed to small peptides.

Nutritional Requirements for Growth and Yeastlike Development of Mucor rouxii Under Carbon Dioxide