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To evaluate the role ofYersinia outer proteins (Yops) in conferring protective immunity against plague, sixyop loci fromYersinia pestis were individually amplified by PCR, cloned, and expressed inEscherichia coli . The recombinant proteins were purified and injected into mice. Most Yop-vaccinated animals succumbed to infection with either wild-type encapsulatedY. pestis or a virulent, nonencapsulated isogenic variant. Vaccination with YpkA significantly prolonged mean survival time but did not increase overall survival of mice infected with the nonencapsulated strain. The only significant protection against death was observed in YopD-vaccinated mice challenged with the nonencapsulated strain.

infection and immunity

Protective Efficacy of Recombinant <i>Yersinia</i> Outer Proteins against Bubonic Plague Caused by Encapsulated and Nonencapsulated <i>Yersinia pestis</i>

Protective Efficacy of Recombinant Yersinia Outer Proteins against Bubonic Plague Caused by Encapsulated and Nonencapsulated Yersinia pestis