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VlsE, the variable surface antigen ofBorrelia burgdorferi , consists of two invariable domains at the amino and carboxyl termini and one central variable domain. The latter contains six invariable regions, IR1 to IR6 , and six variable regions. In the present study, the antigenicity of all of the invariable regions inB. burgdorferi -infected monkeys, humans, and mice was assessed by peptide-based enzyme-linked immunosorbent assays. Only one invariable region, IR6 , was antigenic in all animals of the three host species. IR2 and IR4 were also antigenic in mice.

Analysis of Antibody Response to Invariable Regions of VlsE, the Variable Surface Antigen of Borrelia burgdorferi